"SHH" Related Products


Recombinant Human SHH, Met-tagged

Cat.No.: SHH-606H
Product Overview: Recombinant Human SHH (Accession # NP_000184) Cys24-Gly197 (Cys24Ile-Ile), fused with an N-terminal Met, was produced in E. coli-derived.
Source: E. coli
Species: Human
Predicted N Terminal: Met
Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with BSA as a carrier protein.
Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.The ED50 for this effect is typically 0.1-0.4 µg/mL.
Molecular Mass: Recombinant Human SHH, Met-tagged has a calculated MW of 20 kDa. In SDS-PAGE migrates as 22 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: SHH sonic hedgehog [ Homo sapiens ]
Official Symbol: SHH
Synonyms: SHH; sonic hedgehog; HLP3, HPE3, sonic hedgehog (Drosophila) homolog , sonic hedgehog homolog (Drosophila); sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3;
Gene ID: 6469
mRNA Refseq: NM_000193
Protein Refseq: NP_000184
MIM: 600725
UniProt ID: Q15465
Chromosome Location: 7q36

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