Recombinant Human SHH, Met-tagged SHH-606H

Recombinant Human SHH, Met-tagged

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Recombinant Human SHH, Met-tagged

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Cat.No. : SHH-606H
Product Overview : Recombinant Human SHH (Accession # NP_000184) Cys24-Gly197 (Cys24Ile-Ile), fused with an N-terminal Met, was produced in E. coli-derived.
Source : E. coli
Species : Human
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with BSA as a carrier protein.
Bio-activity : Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.The ED50 for this effect is typically 0.1-0.4 µg/mL.
Molecular Mass : Recombinant Human SHH, Met-tagged has a calculated MW of 20 kDa. In SDS-PAGE migrates as 22 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : SHH sonic hedgehog [ Homo sapiens ]
Official Symbol : SHH
Synonyms : SHH; sonic hedgehog; HLP3, HPE3, sonic hedgehog (Drosophila) homolog , sonic hedgehog homolog (Drosophila); sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3;
Gene ID : 6469
mRNA Refseq : NM_000193
Protein Refseq : NP_000184
MIM : 600725
UniProt ID : Q15465
Chromosome Location : 7q36

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