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Recombinant Rat Eng, Fc Chimera

Cat.No.: Eng-638R
Product Overview: Recombinant Rat Eng (Accession # NP_001010968) was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Rat
Tag: Fc Chimera
Predicted N Terminal: Glu26
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Rat Endoglin/CD10^5 Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.25-1.25 µg/mL.Measured by its ability to inhibit BMP-10-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.15-0.75 µg/mL.
Molecular Mass: Recombinant Rat Eng, Fc Chimera has a calculated MW of 87.0 kDa (monomer). In SDS-PAGE migrates as 100-115 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Eng endoglin [ Rattus norvegicus ]
Official Symbol: Eng
Synonyms: ENG; endoglin; MGC124909;
Gene ID: 497010
mRNA Refseq: NM_001010968
Protein Refseq: NP_001010968
MIM:

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