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Recombinant Human INSR, His-tagged

Cat.No.: INSR-661H
Product Overview: Recombinant Human INSR (Accession # NP_001073285) His28-Arg750 ( alpha subunit) & Ser751-Lys944, fused with a C-terminal 10-His tag ( beta subunit), was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: His28 ( alpha subunit) & Ser751( beta subunit)
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured by its binding ability in a functional ELISA.When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is typically 0.03-0.15 µg/mL.
Molecular Mass: Recombinant Human INSR, His-tagged has a calculated MW of 82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit). In SDS-PAGE migrates as 122-135 kDa and 33-43 kDa, reducing conditions.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: INSR insulin receptor [ Homo sapiens ]
Official Symbol: INSR
Synonyms: INSR; insulin receptor; CD220; IR; HHF5;
Gene ID: 3643
mRNA Refseq: NM_000208
Protein Refseq: NP_000199
MIM: 147670
UniProt ID: P06213
Chromosome Location: 19p13.3-p13.2

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