Recombinant Rat Dkk1, His-tagged Dkk1-678R

Recombinant Rat Dkk1, His-tagged

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Recombinant Rat Dkk1, His-tagged

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Cat.No. : Dkk1-678R
Product Overview : Recombinant Rat Dkk1 (Accession # NP_001099820) Ser23-His270, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Rat
Tag : His
Predicted N Terminal : Ser23
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ND50 for this effect is typically 0.1 - 0.5 µg/mL in the presence of 10 ng/mL of rmWnt-3a.
Molecular Mass : Recombinant Rat Dkk1, His-tagged has a calculated MW of 27.6 kDa. In SDS-PAGE migrates as 38-42 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Dkk1 dickkopf 1 homolog (Xenopus laevis) [ Rattus norvegicus ]
Official Symbol : Dkk1
Synonyms : DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf-related protein 1; dickkopf homolog 1;
Gene ID : 293897
mRNA Refseq : NM_001106350
Protein Refseq : NP_001099820
MIM :

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