Recombinant Human VEGFA VEGFA-703H

Recombinant Human VEGFA

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Recombinant Human VEGFA

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Cat.No. : VEGFA-703H
Product Overview : Recombinant Human VEGFA (Accession # NP_001165097) Ala27-Arg191 was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source : Baculovirus Sf 21 Insect cells
Species : Human
Predicted N Terminal : Ala27
Form : Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 1-6 ng/mL.
Molecular Mass : Recombinant Human VEGFA has a calculated MW of 19.2 kDa (monomer). In SDS-PAGE migrates as 20-22 kDa, reducing conditions39-42 kDa, non-reducing conditions.
Purity : >97%, by SDS-PAGE with silver staining.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 500 µg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.
Gene Name : VEGFA vascular endothelial growth factor A [ Homo sapiens ]
Official Symbol : VEGFA
Synonyms : VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609;
Gene ID : 7422
mRNA Refseq : NM_001025366
Protein Refseq : NP_001020537
MIM :
UniProt ID : P15692
Chromosome Location : 6p12

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