Active Recombinant Human CLU, His-tagged

• Cat.No.: CLU-722H
• Product Overview: Recombinant Human CLU (Accession # NP_001822) Asp23-Arg227 (beta) & Ser228-Glu449 (alpha), fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ser228 ( alpha chain) & Asp23 ( beta chain)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce clustering of Caki-2 human clear cell carcinoma epithelial cells. Schwochau, G. et al. (1998) Kidney Int. 53:1647.The ED50 for this effect is approximately 0.3 µg/mL in the presence of 25 ng/mL of Recombinant Rat GDNF.
• Molecular Mass: Recombinant Human CLU, His-tagged has a calculated MW of 26.7 kDa ( alpha chain) and 24.2 kDa ( beta chain). In SDS-PAGE migrates as 40 kDa and 39 kDa, under reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CLU clusterin [ Homo sapiens ]
• Official Symbol: CLU
• Synonyms: CLU; clusterin; APOJ, CLI, clusterin (complement lysis inhibitor, SP 40,40, sulfated glycoprotein 2, testosterone repressed prostate message 2, apolipoprotein J); apolipoprotein J; complement lysis inhibitor; KUB1; SGP 2; SP 40; sulfated glycoprotein 2; testosterone repressed prostate message 2; TRPM 2; ku70-binding protein 1; aging-associated protein 4; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2; CLI; AAG4; APOJ; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; MGC24903
• Gene ID: 1191
• mRNA Refseq: NM_001831
• Protein Refseq: NP_001822
• MIM: 185430
• UniProt ID: P10909
• Chromosome Location: 8p21-p12