Recombinant Human CD274 protein, mouse IgG1 Fc-tagged, low endotoxin (HPLC-verified)

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Recombinant Human CD274 protein, mouse IgG1 Fc-tagged, low endotoxin (HPLC-verified)

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Cat.No. : CD274-2146H
Product Overview : Recombinant Human CD274 protein (Phe19-Arg238)(low endotoxin, HPLC-verified), fused to Mouse IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Source : HEK293
Species : Human
Tag : Mouse IgG1 Fc
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 51.6 kDa. As a result of glycosylation, the protein migrates as 65-85 kDa under reducing (R) condition, and 130-170 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : 220
Endotoxin : Less than 0.1 EU per ug by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD274-2146H :

Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

SEC-HPLC of CD274-2146H :

The purity of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) was more than 90% as determined by SEC-HPLC.

Bioactivity-ELISA of CD274-2146H :

Immobilized Human PD-1, Tag Free at 0.2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Nivolumab and 1 μg/mL (100 μL/well) Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) with a half maximal inhibitory concentration (IC50) of 0.30 μg/mL (QC tested).

Immobilized Human PD-1, Tag Free, Full Length at 0.2 μg/mL (100 μL/well) can bind Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) with a linear range of 5-156 ng/mL (Routinely tested).

Serial dilutions of nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified): Biotinylated Human PD-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.3789 μg/mL (Routinely tested).

Bioactivity-FACS of CD274-2146H :

Flow Cytometry assay shows that Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 0.1 μg/mL (Routinely tested).

FACS analysis shows that the binding of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-1 antibody. The concentration of PD-L1 used is 1 μg/mL. The IC50 is 0.145 μg/mL (Routinely tested).

Gene Name : CD274
Official Symbol : CD274
Synonyms : CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
Gene ID : 29126
mRNA Refseq : NM_014143
Protein Refseq : NP_054862
MIM : 605402
UniProt ID : Q9NZQ7
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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