Recombinant Mouse Cd274 Protein, His-tagged, Alexa Fluor 555 conjugated
Cat.No. : | Cd274-2275MAF555 |
Product Overview : | Alexa Fluor 555 conjugated recombinant Mouse Cd274 (Phe19-Thr238) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293). |
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Description : | Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. |
Source : | HEK293 |
Species : | Mouse |
Tag : | His |
Form : | Lyophilized |
Molecular Mass : | The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Phe19-Thr238 |
N-terminal Sequence Analysis : | Phe 19 |
Endotoxin : | < 1.0 EU/ μg by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | Cd274 |
Official Symbol : | Cd274 |
Synonyms : | CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik |
Gene ID : | 60533 |
mRNA Refseq : | NM_021893 |
Protein Refseq : | NP_068693 |
UniProt ID : | Q9EP73 |
Products Types
◆ Recombinant Protein | ||
CD274-714M | Recombinant Mouse CD274 Protein, IgG2a Fc-tagged | +Inquiry |
CD274-711H | Recombinant Human CD274 Protein, Strep-tagged | +Inquiry |
CD274-4380C | Recombinant Canine CD274 Protein | +Inquiry |
CD274-1065C | Recombinant Cynomolgus/Rhesus CD274 protein(Met1-Thr239), His-tagged, Biotinylated | +Inquiry |
CD274-199C | Recombinant Human CD274 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
CD274-002RCL | Recombinant Rat CD274 cell lysate | +Inquiry |
CD274-2642MCL | Recombinant Mouse CD274 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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