Active Recombinant Human THOP1 Protein, His-tagged

• Cat.No.: THOP1-528H
• Product Overview: Recombinant Human THOP1 (Accession # NP_003240) Lys2-Cys689, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in MES, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH or Mca-PLGPK(Dnp)-OH.The specific activity is >250 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human THOP1, His-tagged has a calculated MW of 80 kDa. In SDS-PAGE migrates as 80 kDa, reducing conditions.
• Protein length: Lys2-Cys689
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: THOP1 thimet oligopeptidase 1 [ Homo sapiens ]
• Official Symbol: THOP1
• Synonyms: THOP1; thimet oligopeptidase 1; thimet oligopeptidase; endopeptidase 24.15; TOP; MP78; EP24.15; MEPD_HUMAN
• Gene ID: 7064
• mRNA Refseq: NM_003249
• Protein Refseq: NP_003240
• MIM: 601117
• UniProt ID: P52888
• Chromosome Location: 19p13.3