Recombinant Human CDH1 Protein, Fc Chimera CDH1-548H

Recombinant Human CDH1 Protein, Fc Chimera

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Recombinant Human CDH1 Protein, Fc Chimera

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Cat.No. : CDH1-548H
Product Overview : Recombinant Human CDH1 (Accession # NP_004351), fused with , was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : Fc
Predicted N Terminal : Asp155
Form : Lyophilized from a 0.2 µm filtered solution in Tris-Citrate, NaCl and CaCl2.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.When 5 x 10^4 cells/well are added to Recombinant Human E-Cadherin Fc Chimera coated plates (1.5 µg/mL with 100 µL/well), >60% cells will adhere after 90 minutes at 37 °C.Optimal concentration depends on cell type as well as the application or research objectives.
Molecular Mass : Recombinant Human CDH1, Fc Chimera has a calculated MW of 87.7 kDa (monomer). In SDS-PAGE migrates as 120 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : CDH1 cadherin 1, type 1, E-cadherin (epithelial) [ Homo sapiens ]
Official Symbol : CDH1
Synonyms : CDH1; cadherin 1, type 1, E-cadherin (epithelial); UVO; cadherin-1; CD324; E Cadherin; uvomorulin; CAM 120/80; E-Cadherin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; CDHE; ECAD; LCAM; Arc-1;
Gene ID : 999
mRNA Refseq : NM_004360
Protein Refseq : NP_004351
MIM : 192090
UniProt ID : P12830
Chromosome Location : 16q22.1

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