"CD80" Related Products

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Recombinant Human CD80 protein, His/Fc-tagged

Cat.No.: CD80-558H
Product Overview: Recombinant Human CD80 (Val35-Asn242) fused with His tag at C-terminal and Fc of Human IgG1 was expressed in NS0.
Source: NS0
Species: Human
Tag: His
Predicted N Terminal: Val35
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is typically 0.025‑0.15 µg/mL in the presence of PHA.
Molecular Mass: Predicted Molecular Mass: 51.3 (monomer) kDa;SDS-PAGE: 70-90 kDa, reducing conditions.
Protein length: Val35-Asn242
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name: CD80 CD80 molecule [ Homo sapiens ]
Official Symbol: CD80
Synonyms: CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) , CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1;
Gene ID: 941
mRNA Refseq: NM_005191
Protein Refseq: NP_005182
MIM: 112203
UniProt ID: P33681
Chromosome Location: 3q13.3-q21
Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem;
Function: coreceptor activity; protein binding; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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