Active Recombinant Human CHST4 Protein, His-tagged

• Cat.No.: CHST4-561H
• Product Overview: Recombinant Human CHST4 (Accession # NP_005760) His29-His386, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to N-acetyl-D-glucosamine.The specific activity is >150 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CHST4, His-tagged has a calculated MW of 43 kDa. In SDS-PAGE migrates as 45 kDa, reducing conditions.
• Protein length: His29-His386
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 250 µg/mL in PBS.

Gene Information

• Gene Name: CHST4 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4 [ Homo sapiens ]
• Official Symbol: CHST4
• Synonyms: CHST4; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; carbohydrate sulfotransferase 4; HEC GLCNAC 6 ST; LSST; GST-3; gn6st-2; glcNAc6ST-2; HEC-GlcNAc6ST; L-selectin ligand sulfotransferase; N-acetylglucosamine 6-O-sulfotransferase 2; high endothelial cells N-acetylglucosamine 6-O-sulfotransferase; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; GST3; GlcNAc6ST2; HECGLCNAC6ST
• Gene ID: 10164
• mRNA Refseq: NM_001166395
• Protein Refseq: NP_001159867
• UniProt ID: Q8NCG5
• Chromosome Location: 16q22.2