Recombinant Human GLO1 Protein, Met & His-tagged


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Recombinant Human GLO1 Protein, Met & His-tagged

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Cat.No. : GLO1-574H
Product Overview : Recombinant Human GLO1 (Accession # NP_006699) Ala2-Met184, fused with an N-terminal Met and 6-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : & His
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in Tris-HCl and DTT.
Bio-activity : Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione. The specific activity is >100 nmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human GLO1, Met & His-tagged has a calculated MW of 22 kDa. In SDS-PAGE migrates as 25 kDa, reducing conditions.
Protein length : Ala2-Met184
Purity : >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : GLO1 glyoxalase I [ Homo sapiens ]
Official Symbol : GLO1
Synonyms : GLO1; glyoxalase I; lactoylglutathione lyase; GLOD1; glyoxalase domain containing 1; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; S-D-lactoylglutathione methylglyoxal lyase; GLYI;
Gene ID : 2739
mRNA Refseq : NM_006708
Protein Refseq : NP_006699
MIM : 138750
UniProt ID : Q04760
Chromosome Location : 6p21.3-p21.1
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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