Recombinant Human GLO1 Protein, Met & His-tagged GLO1-574H

Recombinant Human GLO1 Protein, Met & His-tagged

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Recombinant Human GLO1 Protein, Met & His-tagged

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Cat.No. : GLO1-574H
Product Overview : Recombinant Human GLO1 (Accession # NP_006699) Ala2-Met184, fused with an N-terminal Met and 6-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : & His
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in Tris-HCl and DTT.
Bio-activity : Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione. The specific activity is >100 nmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human GLO1, Met & His-tagged has a calculated MW of 22 kDa. In SDS-PAGE migrates as 25 kDa, reducing conditions.
Protein length : Ala2-Met184
Purity : >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : GLO1 glyoxalase I [ Homo sapiens ]
Official Symbol : GLO1
Synonyms : GLO1; glyoxalase I; lactoylglutathione lyase; GLOD1; glyoxalase domain containing 1; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; S-D-lactoylglutathione methylglyoxal lyase; GLYI;
Gene ID : 2739
mRNA Refseq : NM_006708
Protein Refseq : NP_006699
MIM : 138750
UniProt ID : Q04760
Chromosome Location : 6p21.3-p21.1

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