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Recombinant Mouse Cdh6 Protein, His-tagged

Cat.No.: Cdh6-593M
Product Overview: Recombinant Mouse Cdh6 (Accession # NP_031692) Met1-Ala615, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Mouse
Tag: His
Predicted N Terminal: Asn22 (pro-protein) & Ser54 (mature protein)
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells.The ED50 for this effect is typically 0.2 - 1.0 µg/mL after a 30 minutes incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass: Recombinant Mouse Cdh6, His-tagged has a calculated MW of 67.0 kDa (pro-protein) & 63.3 kDa (mature protein). In SDS-PAGE migrates as 90-105 kDa, reducing conditions.
Protein length: Met1-Ala615
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: Cdh6 cadherin 6 [ Mus musculus ]
Official Symbol: Cdh6
Synonyms: CDH6; cadherin 6; cadherin-6; kidney cadherin; cad6; K-cadherin; MGC129338; MGC129360;
Gene ID: 12563
mRNA Refseq: NM_007666
Protein Refseq: NP_031692
MIM:

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