||Recombinant Mouse Lefty1 (Accession # NP_034224), fused with , was produced in Mouse myeloma cell line, NS0-derived.
|Predicted N Terminal:
||Leu22 (pro) & His (mature)
||Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
||Measured by its ability to cause heart reversals when injected into the right or left lateral plate mesoderm of Xenopus neurulae embryos.Between 10-50 ng per embryo will cause 50% heart reversals.
||Recombinant Mouse Lefty1 has a calculated MW of 26.9 kDa. In SDS-PAGE migrates as 12 kDa and 36 kDa, reducing conditions.
||>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).