Active Recombinant Mouse Il10 protein

• Cat.No.: Il10-642M
• Product Overview: Recombinant Mouse Il10 protein(NP_034678.1)(Ser19-Ser178) was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: Ser19-Ser178
• Form: Lyophilized from sterile 200mM NaCl, 20mM Tris, pH 7.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is typically 0.5-4 ng/mL.
• Molecular Mass: The recombinant mouse IL10 consists of 161 amino acids and predicts a molecular mass of 18.9 kDa.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il10 interleukin 10 [ Mus musculus ]
• Official Symbol: Il10
• Synonyms: IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; Il-10
• Gene ID: 16153
• mRNA Refseq: NM_010548
• Protein Refseq: NP_034678