Active Recombinant Mouse Elane Protein, His-tagged

• Cat.No.: Elane-694M
• Product Overview: Recombinant Mouse Elane (Accession # NP_056594) Ser27-Arg260, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ser27
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC). The specific activity is >500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Elane, His-tagged has a calculated MW of 27 kDa. In SDS-PAGE migrates as 34 kDa, reducing conditions.
• Protein length: Ser27-Arg260
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Elane elastase, neutrophil expressed [ Mus musculus ]
• Official Symbol: Elane
• Synonyms: ELANE; elastase, neutrophil expressed; neutrophil elastase; elastase-2; leukocyte elastase; elastase 2, neutrophil; NE; Ela2; F430011M15Rik
• Gene ID: 50701
• mRNA Refseq: NM_015779
• Protein Refseq: NP_056594