Recombinant Mouse Elane (Accession # NP_056594) Ser27-Arg260, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source :
Mammalian cells
Species :
Mouse
Tag :
His
Predicted N Terminal :
Ser27
Form :
Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity :
Measured by its ability to cleave the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC). The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Molecular Mass :
Recombinant Mouse Elane, His-tagged has a calculated MW of 27 kDa. In SDS-PAGE migrates as 34 kDa, reducing conditions.
Protein length :
Ser27-Arg260
Purity :
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage :
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.