Active Recombinant Human PLA2G4A Protein, His-tagged

• Cat.No.: PLA2G4A-752H
• Product Overview: Recombinant Human PLA2G4A (Accession # NP_077734) Met1-Ala749, fused with a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ser2
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol, Brij-35 and DTT.
• Bio-activity: Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine.The specific activity is >30 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human PLA2G4A, His-tagged has a calculated MW of 86 kDa. In SDS-PAGE migrates as 85-95 kDa, reducing conditions.
• Protein length: Met1-Ala749
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: PLA2G4A phospholipase A2, group IVA (cytosolic, calcium-dependent) [ Homo sapiens ]
• Official Symbol: PLA2G4A
• Synonyms: PLA2G4A; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4; cytosolic phospholipase A2; cPLA2 alpha; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein; cPLA2-alpha; MGC126350
• Gene ID: 5321
• mRNA Refseq: NM_024420
• Protein Refseq: NP_077734
• UniProt ID: P47712
• Chromosome Location: 1q25