Recombinant Mouse Sost Protein, His-tagged Sost-753M

Recombinant Mouse Sost Protein, His-tagged


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Recombinant Mouse Sost Protein, His-tagged

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Cat.No. : Sost-753M
Product Overview : Recombinant Mouse Sost (Accession # NP_077769) Gln24-Tyr211, fused with an N-terminal 7-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Mouse
Tag : His
Predicted N Terminal : His
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 1-5 µg/mL in the presence of Recombinant Mouse Wnt-3a.
Molecular Mass : Recombinant Mouse Sost, His-tagged has a calculated MW of 22 kDa. In SDS-PAGE migrates as 30 kDa, reducing conditions.
Protein length : Gln24-Tyr211
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Sost sclerostin [ Mus musculus ]
Official Symbol : Sost
Synonyms : SOST; sclerostin; 5430411E23Rik;
Gene ID : 74499
mRNA Refseq : NM_024449
Protein Refseq : NP_077769

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