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Recombinant Mouse Sost Protein, His-tagged

Cat.No.: Sost-753M
Product Overview: Recombinant Mouse Sost (Accession # NP_077769) Gln24-Tyr211, fused with an N-terminal 7-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Mouse
Tag: His
Predicted N Terminal: His
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 1-5 µg/mL in the presence of Recombinant Mouse Wnt-3a.
Molecular Mass: Recombinant Mouse Sost, His-tagged has a calculated MW of 22 kDa. In SDS-PAGE migrates as 30 kDa, reducing conditions.
Protein length: Gln24-Tyr211
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: Sost sclerostin [ Mus musculus ]
Official Symbol: Sost
Synonyms: SOST; sclerostin; 5430411E23Rik;
Gene ID: 74499
mRNA Refseq: NM_024449
Protein Refseq: NP_077769
MIM:

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