Active Recombinant Mouse Pvrl4 Protein, His-tagged

• Cat.No.: Pvrl4-764M
• Product Overview: Recombinant Mouse Pvrl4 (Accession # NP_082169) Tyr28-Ile349, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Tyr28
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 x 10^4 cells/well are added to recombinant mouse Nectin-4 coated plates (10 µg/mL with 100 µL/well), approximately 45% - 65% will adhere after 1 hour incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Pvrl4, His-tagged has a calculated MW of 35.6 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing condtions.
• Protein length: Tyr28-Ile349
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Pvrl4 poliovirus receptor-related 4 [ Mus musculus ]
• Official Symbol: Pvrl4
• Synonyms: PVRL4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; nectin 4; nectin-4; ig superfamily receptor LNIR; Prr4; 1200017F15Rik
• Gene ID: 71740
• mRNA Refseq: NM_001122680
• Protein Refseq: NP_001116152