Active Recombinant Mouse Klb Protein, His-tagged

• Cat.No.: Klb-778M
• Product Overview: Recombinant Mouse Klb (Accession # NP_112457) Phe53-Leu995, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Phe53
• Form: Supplied as a 0.2 µm filtered solution in PBS, Glycerol and EDTA.
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGF RIIIc.The ED50 for this effect is typically 0.2-1 µg/mL in the presence of rhFGF-21.Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells.The ED50 for this effect is typically 0.75-3 µg/mL in the presence of rhFGF-21.
• Molecular Mass: Recombinant Mouse Klb, His-tagged has a calculated MW of 110.2 kDa. In SDS-PAGE migrates as 140 kDa, reducing conditions.
• Protein length: Phe53-Leu995
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Klb klotho beta [ Mus musculus ]
• Official Symbol: Klb
• Synonyms: KLB; klotho beta; beta-klotho; BKL; klotho beta-like protein; AV071179; betaKlotho
• Gene ID: 83379
• mRNA Refseq: NM_031180
• Protein Refseq: NP_112457