Active Recombinant Human IL36B

• Cat.No.: IL36B-852H
• Product Overview: Recombinant Human IL36B (Accession # NP_775270) Arg5-Glu157, fused with , was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Arg5
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce IL-8 secretion in human preadipocytes. van Asseldonk, E.J. et al. (2010) Obesity 18:2234.The ED50 for this effect is typically 2 - 12 ng/mL.
• Molecular Mass: Recombinant Human IL36B has a calculated MW of 17.2 kDa. In SDS-PAGE migrates as 17 kDa, reducing conditions.
• Protein length: Arg5-Glu157
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: IL36B interleukin 36, beta [ Homo sapiens ]
• Official Symbol: IL36B
• Synonyms: IL36B; interleukin 36, beta; IL1F8, interleukin 1 family, member 8 (eta); interleukin-36 beta; FIL1; FILI (ETA); IL 1F8; IL 1H2; IL1 ETA; IL1H2; MGC126880; MGC126882; FIL1 eta; IL-1 eta; IL-1F8 (FIL1-eta); interleukin-1 eta; interleukin 1, eta; interleukin-1 homolog 2; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin-1 family member 8; IL1F8 (Canonical product IL-1F8a); interleukin 1 family, member 8 (eta); FIL1H; IL1F8; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
• Gene ID: 27177
• mRNA Refseq: NM_014438
• Protein Refseq: NP_055253
• MIM: 605508
• UniProt ID: Q9NZH7
• Chromosome Location: 2q14