Active Recombinant Mouse Vsig4 Protein, Fc Chimera

• Cat.No.: Vsig4-861M
• Product Overview: Recombinant Mouse Vsig4 (Accession # NP_808457), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: His20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of splenic T lymphocytes. Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.The presence of immobilized rmVSIG4/Fc Chimera inhibited the anti-CD3 response by 50% - 70%. The ED50 for this effect is typically 1.25 - 5 µg/mL.Measured by its ability to bind human iC3b in a functional ELISA. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Vsig4, Fc Chimera has a calculated MW of 46 kDa (monomer). In SDS-PAGE migrates as 60-65 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Vsig4 V-set and immunoglobulin domain containing 4 [ Mus musculus ]
• Official Symbol: Vsig4
• Synonyms: VSIG4; V-set and immunoglobulin domain containing 4; V-set and immunoglobulin domain-containing protein 4; complement receptor of the immunoglobulin superfamily; CRIg; Z39IG; BC025105; A530061A11; MGC36211
• Gene ID: 278180
• mRNA Refseq: NM_177789
• Protein Refseq: NP_808457