Active Recombinant Human FCN1 Protein, His-tagged

• Cat.No.: FCN1-894H
• Product Overview: Recombinant Human FCN1 (Accession # O00602) Gln28-Ala326, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala30
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl.
• Bio-activity: Measured by its ability to bind immobilized Fetuin in a functional ELISA. Liu, Y. et al. (2005) J. Immunol. 175:3150.The ED50 for this effect is typically 20 - 50 ng/mL in the presence of 0.4 ng/mL of rhTGF-beta 2.
• Molecular Mass: Recombinant Human FCN1, His-tagged has a calculated MW of 33.4 kDa. In SDS-PAGE migrates as 35-36 kDa, reducing conditions.
• Protein length: Gln28-Ala326
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: FCN1 ficolin (collagen/fibrinogen domain containing) 1 [ Homo sapiens ]
• Official Symbol: FCN1
• Synonyms: FCN1; ficolin (collagen/fibrinogen domain containing) 1; ficolin-1; FCNM; M-ficolin; ficolin-A; ficolin-alpha; collagen/fibrinogen domain-containing protein 1; ficolin (collagen/fibrinogen domain-containing) 1
• Gene ID: 2219
• mRNA Refseq: NM_002003
• Protein Refseq: NP_001994
• MIM: 601252
• UniProt ID: O00602
• Chromosome Location: 9q34