||Recombinant Human UBE2C (Accession # O00762), fused with an N-terminal Met and 7-His tag, was produced in E. coli.
||X mg/ml (X µM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
||Recombinant Human His6-UbcH10/UBE2C is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH10/UBE2C concentration of 0.1-1 µM.
||Recombinant Human UBE2C, Met & His-tagged has a calculated MW of 21 kDa.
||>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).