Recombinant Human UBE2C Protein, Met & His-tagged UBE2C-899H

Recombinant Human UBE2C Protein, Met & His-tagged

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Recombinant Human UBE2C Protein, Met & His-tagged

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Cat.No. : UBE2C-899H
Product Overview : Recombinant Human UBE2C (Accession # O00762), fused with an N-terminal Met and 7-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : & His
Form : X mg/ml (X µM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Bio-activity : Recombinant Human His6-UbcH10/UBE2C is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH10/UBE2C concentration of 0.1-1 µM.
Molecular Mass : Recombinant Human UBE2C, Met & His-tagged has a calculated MW of 21 kDa.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : UBE2C ubiquitin-conjugating enzyme E2C [ Homo sapiens ]
Official Symbol : UBE2C
Synonyms : UBE2C; ubiquitin-conjugating enzyme E2C; ubiquitin-conjugating enzyme E2 C; UBCH10; ubiquitin-protein ligase C; ubiquitin carrier protein C; ubiquitin carrier protein E2-C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; dJ447F3.2;
Gene ID : 11065
mRNA Refseq : NM_007019
Protein Refseq : NP_008950
MIM : 605574
UniProt ID : O00762
Chromosome Location : 20q13.12

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