Active Recombinant Mouse Sema6b Protein, Fc Chimera

• Cat.No.: Sema6b-987M
• Product Overview: Recombinant Mouse Sema6b (Accession # O54951), fused with , was produced in Human embryonic kidney cell, HEK293-derived.

Specification

• Source: HEK293 Cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Leu27
• Form: Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and Tween® 20.
• Bio-activity: Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones.5 µg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta -NGF.
• Molecular Mass: Recombinant Mouse Sema6b, Fc Chimera has a calculated MW of 91 kDa (monomer). In SDS-PAGE migrates as 100-115 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Sema6b sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B [ Mus musculus ]
• Official Symbol: Sema6b
• Synonyms: SEMA6B; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; semaphorin-6B; sema N; sema VIB; semaphorin-N; semaphorin VIB; VIb; Sema; Seman; semaZ; MGC143995; MGC143996
• Gene ID: 20359
• mRNA Refseq: NM_001130456
• Protein Refseq: NP_001123928