Active Recombinant Mouse Dsg2 Protein, Fc Chimera

• Cat.No.: Dsg2-990M
• Product Overview: Recombinant Mouse Dsg2 (Accession # O55111), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Leu29 & Ala55
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.When 3 x 10^4 cells are added to Recombinant Mouse Desmoglein-2 Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 30%-60% will adhere after 1 hour at 37 °C.Optimal dilutions should be determinded by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Dsg2, Fc Chimera has a calculated MW of 92.9 kDa (monomer). In SDS-PAGE migrates as 100-120 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Dsg2 desmoglein 2 [ Mus musculus ]
• Official Symbol: Dsg2
• Synonyms: DSG2; desmoglein 2; desmoglein-2; AA408168; D18Ertd293e
• Gene ID: 13511
• mRNA Refseq: NM_007883
• Protein Refseq: NP_031909