Active Recombinant Zebrafish efnb2a Protein, Fc Chimera

• Cat.No.: efnb2a-1021Z
• Product Overview: Recombinant Zebrafish efnb2a (Accession # O73874), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Zebrafish
• Tag: Fc
• Predicted N Terminal: Leu25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to compete with biotinylated rmEphrin-B2/Fc Chimera for binding with immobilized rmEph-B2/Fc Chimera in a functional ELISA assay. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Zebrafish efnb2a, Fc Chimera has a calculated MW of 48.5 kDa (monomer). In SDS-PAGE migrates as 60-70 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 500 µg/mL in PBS.

Gene Information

• Gene Name: efnb2a ephrin-B2a [ Danio rerio (zebrafish) ]
• Official Symbol: efnb2a
• Synonyms: efnb2; id:ibd5072; ephrin-B2a; ephrin-B2a; ephrin B2a
• Gene ID: 30219
• mRNA Refseq: NM_131023.1
• Protein Refseq: NP_571098.1
• UniProt ID: O73874
• Chromosome Location: chromosome: 9