Species : |
Rat |
Source : |
E.coli |
Tag : |
Non |
Protein Length : |
Asn299-Ser407 |
Predicted N Terminal : |
Met |
Form : |
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Bio-activity : |
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is typically 0.8-4.8 ng/mL. |
Molecular Mass : |
Predicted Molecular Mass12.6 (monomer) kDa |
Endotoxin : |
<0.01 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">0.01> |
Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : |
Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
Shipping : |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |