Active Recombinant Human SYVN1, His & SUMO-tagged

• Cat.No.: SYVN1-109H
• Product Overview: Recombinant Human RFFL, fused with His6-SUMO, was expressed in E.coli. This construct is a Hrd1 N-terminal deletion containing residues 236-617 consisting of the complete C-terminal cytoplasmic portion. The N-terminal transmembrane spanning portion has been deleted. This Hrd1 N-terminal deletion mutant is active as an E3 ligase containing the RING and substrate recognition domains.

Specification

• Source: E.coli
• Species: Human
• Tag: His & SUMO
• Form: Dissolved in PBS, 10% glycerol
• Bio-activity: Typical enzyme concentration 20nM -1 μM is used for in vitro conjugation depending on assay conditions.
• Molecular Mass: 39.3 kDa (without SUMO tag)
• Storage: Store at -80℃. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: SYVN1 synovial apoptosis inhibitor 1, synoviolin [ Homo sapiens ]
• Official Symbol: SYVN1
• Synonyms: SYVN1; synovial apoptosis inhibitor 1, synoviolin; E3 ubiquitin-protein ligase synoviolin; HRD1; KIAA1810; MGC40372
• Gene ID: 84447
• mRNA Refseq: NM_032431
• Protein Refseq: NP_115807
• MIM: 608046
• UniProt ID: Q86TM6
• Chromosome Location: 11q13
• Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; HRD1/SEL1 ERAD complex, organism-specific biosystem; HRD1/SEL1 ERAD complex, conserved biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem
• Function: acid-amino acid ligase activity; ligase activity; metal ion binding; protein binding; zinc ion binding