Recombinant Rhesus macaque TNFRSF4 protein, Fc-tagged

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Recombinant Rhesus macaque TNFRSF4 protein, Fc-tagged

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Cat.No. : TNFRSF4-102M
Product Overview : Recombinant Rhesus macaque TNFRSF4 protein (Lys28-Ala214), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40/TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4/OX40L/GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
Source : HEK293
Species : Rhesus macaque
Tag : human IgG1 Fc
Form : Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 46.9 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE).
Protein length : 187
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of TNFRSF4-102M :

Rhesus macaque OX40, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of TNFRSF4-102M :

Immobilized Human OX40 Ligand, His Tag at 5 μg/mL (100 μl/well) can bind Rhesus macaque OX40, Fc Tag with a linear range of 1-20 ng/mL (QC tested).

Gene Name : TNFRSF4
Official Symbol : TNFRSF4
Synonyms : OX40, CD134, OX40L receptor, ACT35, TXGP1L
Gene ID : 699674
mRNA Refseq : XM_001090870.4
Protein Refseq : XP_001090870.1
UniProt ID : A0A5F7ZEC3

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