Active Recombinant Human CASP9 protein
Cat.No. : | CASP9-49H |
Product Overview : | Recombinant Human CASP9 was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | Caspase-9 is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger activation of downstream effector caspases, leading to disassembly of cell structures. |
Source : | E. coli |
Species : | Human |
Form : | Lyophilized powder |
Bio-activity : | > 400 units/mg |
Purity : | ≥90% |
Unit Definition : | One unit of the recombinant caspase-9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37 centigrade in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT. |
Applications : | Active caspase-9 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity. |
Notes : | Centrifuge the vial prior to opening. |
Storage : | The lyophilized caspase-9 is stable for 1 year at –70 centigrade. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70 centigrade. Avoid multiple freeze/thaw cycles as activity might decrease. |
Reconstitution : | Reconstitute to 1 unit per μl in PBS containing 15% glycerol. |
Gene Name : | CASP9 caspase 9, apoptosis-related cysteine peptidase [ Homo sapiens ] |
Official Symbol : | CASP9 |
Synonyms : | CASP9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis related cysteine protease; caspase-9; APAF 3; ICE LAP6; MCH6; PPP1R56; protein phosphatase 1; regulatory subunit 56; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56; APAF3; APAF-3; ICE-LAP6; CASPASE-9c; |
Gene ID : | 842 |
mRNA Refseq : | NM_001229 |
Protein Refseq : | NP_001220 |
MIM : | 602234 |
UniProt ID : | P55211 |
Chromosome Location : | 1p36.21 |
Pathway : | AKT phosphorylates targets in the cytosol, organism-specific biosystem; Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; |
Function : | cysteine-type endopeptidase activity; enzyme activator activity; peptidase activity; protein binding; |
Products Types
◆ Recombinant Protein | ||
Casp9-773M | Recombinant Mouse Casp9 Protein, MYC/DDK-tagged | +Inquiry |
CASP9-2596H | Recombinant Human CASP9 Protein, His (Fc)-Avi-tagged | +Inquiry |
CASP9-1248M | Recombinant Mouse CASP9 Protein, His (Fc)-Avi-tagged | +Inquiry |
CASP9-0433H | Recombinant Human CASP9 Protein, GST-Tagged | +Inquiry |
CASP9-627H | Recombinant Human CASP9 protein, His-tagged | +Inquiry |
◆ Lysates | ||
CASP9-7828HCL | Recombinant Human CASP9 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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