Recombinant Rat Nme2 protein, His-tagged

• Cat.No.: Nme2-33R
• Product Overview: Recombinant rat Nme2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Nme2 also known as nucleoside diphosphate kinaseB is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. Nme2 is a ubiquitous enzyme that catalyses phosphorylation of nucleoside 5´-diphosphate (NDP) to the corresponding triphosphate (NTP), following a Ping-Pong mechanism which includes the formation of a phosphohistidine intermediate.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline pH 7.4 contaning 10% glycerol
• Molecular Mass: 19.7kDa (175aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMANLERT FIAIKPDGVQ RGLVGEIIKR FEQKGFRLVA MKFLRASEEH LKQHYIDLKD RPFFPGLVKYMNSGPVVAMV WEGLNVVKTG RVMLGETNPA DSKPGTIRGD FCIQVGRNII HGSDSVESAE KEIGLWFKPE ELIDYKSCAH DWVYE
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Nme2 non-metastatic cells 2, protein (NM23B) expressed in [ Rattus norvegicus ]
• Official Symbol: Nme2
• Synonyms: NME2; non-metastatic cells 2, protein (NM23B) expressed in; nucleoside diphosphate kinase B; P18; NDK B; NDP kinase B; NDP kinase alpha; histidine protein kinase NDKB; expressed in non-metastatic cells 2; NDKB; nm23-2; p18-12d
• Gene ID: 83782
• mRNA Refseq: NM_031833
• Protein Refseq: NP_114021
• Pathway: Metabolic pathways, organism-specific biosystem; Nucleotide Metabolism, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem; Pyrimidine ribonucleotide biosynthesis, UMP =>
• Function: ATP binding; drug binding; enzyme binding; fatty acid binding; intermediate filament binding; kinase activity; metal ion binding; nucleoside diphosphate kinase activity; nucleoside diphosphate kinase activity; nucleotide binding; protein histidine kinase