Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human IGFBP3 protein

Cat.No. : IGFBP3-80H
Product Overview : Recombinant Human IGFBP3 protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
  • Related Products
Description : Insulin-like Growth Factor-Binding Protein 3 (IGF-BP3) belongs to the IGFBP family, which are all cysteinerich proteins with conserved cysteine and have an IGFBP domain and a thyroglobulin type-I domain. Mature human IGF-BP3 contains 264 a.a. with three potential N-linked and two potential O-linked glycosylation sites. It is expressed by most tissues and has higher levels during extrauterine life and peak during puberty. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF. The protein forms a ternary complex with IGF-I or II and acid-labile subunit. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Decreased plasma levels of IGF-BP3 often happen during the progression of prostate cancer from benign to metastatic disease.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 × 10³ IU/mg in the presence of 15 ng/ml of rHuIGF-II.
Molecular Mass : Approximately 28.8 kDa, a single non-glycosylated polypeptide chain containing 264 amino acids.
Protein length : 264
AA Sequence : GASSGGLGPVVRCEPCDARALAQCA PPPAVCAELVREPGCGCCLTCALSE GQPCGIYTERCGSGLRCQPSPDEAR PLQALLDGRGLCVNASAVSRLRAYL LPAPPAPGNASESEEDRSAGEVESP SVSSTHRVSDPKFHPLHSKIIIIKK GHAKDSQRYKVDYESQSTDTQNFSS ESKRETEYGPCRREMEDTLNHLKFL NVLSPRGVHIPNCDKKGFYKKKQCR PSKGRKRGFCWCVDKYGQPLPGYTT KGKEDVHCYSMQSK
Endotoxin : Less than 1 EU/μg of rHuIGF-BP3 as determined by LAL method.
Purity : >98% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : IGFBP3
Official Symbol : IGFBP3
Synonyms : IGFBP3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP 53; growth hormone dependent binding protein; IBP3; IGF binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; growth hormone-dependent binding protein; BP-53;
Gene ID : 3486
mRNA Refseq : NM_000598
Protein Refseq : NP_000589
MIM : 146732
UniProt ID : P17936

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Are there any genetic factors that influence IGFBP3 protein levels? 08/11/2022

Yes, genetic variations can affect IGFBP3 expression, potentially leading to differences in protein levels among individuals.

How does IGFBP3 protein influence bone health? 11/24/2021

IGFBP3 is involved in bone metabolism and is associated with bone mineral density. Low levels of IGFBP3 have been linked to decreased bone density and increased risk of fractures.

Are there any side effects associated with therapies targeting IGFBP3? 08/04/2021

The potential side effects of therapies targeting IGFBP3 are still being studied, and it varies depending on the specific treatment approach being used.

How do lifestyle factors such as diet and exercise influence IGFBP3 levels? 04/23/2021

Healthy lifestyle choices, including a balanced diet and regular exercise, can positively influence IGFBP3 levels, promoting overall health and reducing the risk of related diseases.

What are the implications of IGFBP3 deficiency in children's growth and development? 01/14/2021

IGFBP3 deficiency can impact children's growth and development, potentially leading to short stature and delayed puberty, as IGFBP3 is a key regulator of growth hormone actions.

Customer Reviews (3)

Write a review
Reviews
05/04/2020

    A manufacturer that offers customization options can enable the researcher to adhere to their experimental design requirements more effectively.

    01/20/2019

      A reliable manufacturer should be able to provide a consistently pure and high-quality IGFBP3 protein, ensuring that the samples are suitable for research purposes.

      01/02/2019

        This can help ensure compatibility between different components used in the trials and ease the overall experimental workflow.

        Ask a Question for All IGFBP3 Products

        Required fields are marked with *

        My Review for All IGFBP3 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends