| Cat. No. : |
Bax-244M |
| Description : |
The Bcl-2-associated X protein, or Bax is a protein of theBcl-2gene family. It promotesapoptosisby competing withBcl-2proper. Bax is a pro-apoptotic Bcl-2 protein containing BH1, BH2 and BH3 domains. In healthy mammalian cells, the majority of BAX is found in thecytosol, but upon initiation of apoptoticsignaling, Bax undergoes a conformation shift, and inserts intoorganellemembranes, primarily the outermitochondrialmembrane. Bax is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel,VDAC. Alternatively, growing evidence suggest that activated Bax and/orBakform an oligomeric pore,MACin the outer membrane. This result in the release ofcytochrome cand other pro-apoptotic factors from the mitochondria often referred to as mitochondrial outer membrane permeabilization, leading to activation ofcaspases. |
| Source : |
Escherichia Coli. |
| Physical Appearance : |
Sterile Filtered colorless solution. |
| Purity : |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Formulation : |
The protein, 200µg/ml contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. |
| Other Available Varients : |
Recombinant Mouse BID, Recombinant Mouse tBID, Recombinant Mouse GST-BID Recombinant Mouse GST-Bax, Recombinant Mouse GST-Bax BH1, Recombinant Mouse GST-Bax BH2, Recombinant Mouse GST-Bax BH3, Recombinant Mouse GST-Bax IGDE, Recombinant Mouse GST-Bax D/A in IGDE. |
| Endotoxin : |
It is less than 0.1 ng/µg of rmBax. |
| Applications : |
Input marker or positive control (Western Blotting). Function study (binding assay). |
| Stability : |
The rMuBax although stable at 12°Cfor 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
