||Recombinant Human Desmin has a molecular mass of 53,539 dalton and is produced inE.Coli.
||This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||The protein was lyophilized from a 1mg/ml solution containing 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA and 10 mM methylammonium chloride.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||It is recommended to reconstitute the lyophilized rHuDesmin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized rHuDesmin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuDesmin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.