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Recombinant Human Keratin 18

Cat. No.: KRT18-464H
Product Overview: Recombinant Human Cytokeratin 18 produced inE.Coliis a single,non-glycosylated polypeptide chain and has a molecular mass of 53,532 Dalton. The rHuCK-18 is purified by proprietary chromatographic techniques.
Description: Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM EDTA and 10mM methylammonium chloride.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized rHuCK-18 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Dimers And Aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuCK-18.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of CK-18 as a Reference Standard.
Reconstitution To Filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco"s PBS).
Stability: Lyophilized rHuCK-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuCK-18 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: KRT18 keratin 18 [ Homo sapiens ]
Synonyms: KRT18; keratin 18; K18; CYK18;cytokeratin 18; cell proliferation-inducing protein 46;Keratin, type I cytoskeletal 18; Cytokeratin-18; CK-18; Keratin-18; Cell proliferation-inducing gene 46 protein; PIG46; cytokeratin 18
Gene ID: 3875
mRNA Refseq: NM_000224
Protein Refseq: NP_000215
MIM: 148070
UniProt ID: P05783
Chromosome Location: 12q13
Pathway: Pathogenic Escherichia coli infection - EHEC
Function: protein binding; structural molecule activity

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