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Recombinant Human CSF2, His-tagged

Cat.No. : CSF2-143H
Product Overview : rHuman GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrate as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosylation. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Granulocyte-macrophage colony-stimulating factor (GM-CSF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal free product.
Description : GMCSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. It′s involved in differentiation of dendritic cells and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation. Human GMCF is an important therapeutic cytokine used in the treatment of myeloid leukemia, neutropenia and aplastic anemia and it could become interesting in the treatment following bone marrow transplantation. It performs its biological activity by binding to a specific receptor complex which is composed of a cytokine-specific alpha chain and beta chain, shared with the receptors for interleukin-3 and interleukin-5. GMCSR has been identified to mediate the activation of Jak-Stat and MAPK pathways.
Source : Nicotiana benthamiana
Species : Human
Tag : His
Form : Recombinant human GM-CSF is lyophilized from 10 mM PBS buffer pH 7.6 and 0.2 M NaCl.
Bio-activity : The activity of recombinant human GM-CSF is determined by the dose-dependent induction of human TF-1 proliferation cell *Cell proliferation was measured by MTT method. ED50 is ≤ 0.05 ng/mL.
Molecular Mass : rHuman GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrate as a broad band between 15 and 25 kDa due to post-translation modification, in particul
AA Sequence : HHHHHHHHHHAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQG LRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
Endotoxin : < 0.04 eu ug protein (lal>
Purity : >97% by SDS-PAGE gel
Applications : Cell culture, Western Blot.
Storage : This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted rh GM-CSF should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.
Reconstitution : Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng / μl. Optimal concentration should be determined for specific application and cell lines. Optimal reconstitution please follow batch Quality Control sheet instructions.
Gene Name : CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ]
Official Symbol : CSF2
Synonyms : CSF2; colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; GM CSF; GMCSF; granulocyte macrophage colony stimulating factor; molgramostin; sargramostim; CSF; colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897;
Gene ID : 1437
mRNA Refseq : NM_000758
Protein Refseq : NP_000749
MIM : 138960
UniProt ID : P04141
Chromosome Location : 5q23-q31
Pathway : Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function : cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding;

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