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Active Recombinant Human TNFRSF10B, Fc Chimera

Cat.No. : TNFRSF10B-460H
Product Overview : Recombinant Humantumor necrosis factor receptor superfamily, member 10b was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modifiedhuman 293 cells.
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Cat. No. : TNFRSF10B-460H
Description : TRAILR2, also known as death receptor 5, TNFRSF10B and TNF-related apoptosis-inducing ligand receptor 2, is a type 1 transmembrane protein that contains a death domain in its cytoplasmic domain. TRAILR2 is a receptor for the cytotoxic ligand TRAIL/TNFSF10. TRAILR2 is widely expressed in adult and fetal tissues. TRAILR2 is produced as an ECD-Fc fusion protein with the aim of enhancing its activity. ECD-Fc fusion proteins have an advantage over soluble receptors because many receptors are only functional in dimeric form. Fusion to the Fc domain of IgG1 induces dimerization due to the ability of the Fc domain to form disulfide bonds. The resulting dimeric receptor ECD-Fc mimics the activated form of the receptor and possess enhanced affinity for its cognate ligand relative to its monomeric form.
Source : human 293 cells.
Molecular Mass : Under reducing conditions TRAILR1 - Fc Chimera migrates as a broad band between 40 and 50 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified TRAIL R1 - Fc Chimera that has a predicted monomeric molecular mass of 41.5 kDa.
PI : TRAILR2 - Fc Chimera separates into a number of isoforms with a pI between 6.0 and 8.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TRAIL R2 - Fc Chimera that has a predicted pI of 6.5.
% Carbohydrate : TRAILR2 - Fc Chimera consists of 0–25% carbohydrate by weight.
Glycosylation : TRAILR2 - Fc Chimera contains N- and O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE and visualized by silver stain.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
Activity : The ED50of TRAIL R2 - Fc Chimera is typically 38-40 ng/ml as measured by its ability to neutralize TRAIL mediated cytotoxicity using the human leukemic Jurkat cells.
Gene Name : TNFRSF10B tumor necrosis factor receptor superfamily, member 10b [ Homo sapiens ]
Synonyms : tumor necrosis factor receptor superfamily, member 10b; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5; TNFRSF10B; Fas-like protein; death receptor 5; cytotoxic TRAIL receptor-2; apoptosis inducing receptor TRAIL-R2; apoptosis inducing protein TRICK2A/2B; TRAIL receptor 2; TNF-related apoptosis-inducing ligand receptor 2; death domain containing receptor for TRAIL/Apo-2L; tumor necrosis factor receptor-like protein ZTNFR9; p53-regulated DNA damage-inducible cell death receptor(killer); Death receptor 5; OTTHUMP00000123492; CD262 antigen
Gene ID : 8795
mRNA Refseq : NM_003842
Protein Refseq : NP_003833
MIM : 603612
UniProt ID : O14763
Chromosome Location : 8p22-p21
Pathway : Apoptosis; Cytokine-cytokine receptor interaction; Natural killer cell mediated cytotoxicity; p53 signaling pathway; Apoptosis
Function : TRAIL binding; caspase activator activity; receptor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does TNFRSF10B activation induce apoptosis in cancer cells? 03/09/2023

Activation of TNFRSF10B triggers apoptotic pathways, leading to programmed cell death in cancer cells.

Is TNFRSF10B involved in other diseases besides cancer? 04/19/2021

TNFRSF10B has been implicated in certain autoimmune diseases and neurodegenerative disorders, highlighting its diverse roles in various physiological processes.

Can TNFRSF10B be used as a diagnostic marker for cancer? 03/08/2019

TNFRSF10B expression levels may serve as a potential diagnostic marker for certain cancers, aiding in cancer detection and prognosis.

In what types of cancer is TNFRSF10B expression altered? 03/19/2017

TNFRSF10B expression is often altered in various cancers, including breast cancer, colorectal cancer, and lung cancer.

Are there any side effects associated with targeting TNFRSF10B in cancer treatment? 06/06/2016

Side effects may occur, and ongoing research aims to minimize these effects while maximizing the therapeutic benefits of targeting TNFRSF10B.

Customer Reviews (3)

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08/11/2020

    The combination of high-quality TNFRSF10B protein and excellent technical support provided by the manufacturer makes them the ideal choice for my research.

    10/07/2018

      The manufacturer's extensive experience and expertise in the field of TNFRSF10B protein research make them a trusted partner.

      03/04/2017

        The manufacturer's commitment to providing excellent technical support is highly valuable.

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