||Recombinant human VAMP4 protein, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 14.5 kDa (123aa).
||VAMP4, also known as vesicle-associated membrane protein 4, is a member of the synaptobrevin family involved in docking and/or fusion of vesicles with cell membrane. This protein is enriched in the trans-Golgi network and may play a role in trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules.
|Amino Acid Sequence:
||MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, and 20% glycerol.
||> 90% by SDS – PAGE.
||0.5 mg/ml (determined by Bradford assay).
||< 1.0 EU per 1 μg of protein (determined by LAL method ).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.