Recombinant Human TNFSF12 TNFSF12-565H

Recombinant Human TNFSF12


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Recombinant Human TNFSF12

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Cat. No. : TNFSF12-565H
Product Overview : Recombinant human TNFSF12 produced inE. Coliis a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein.
Description : TWEAK belongs to the TNF family of ligands and signals through TWEAKR also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NFkB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-gamma. TWEAK also promotes proliferation and migration of endothelial cells.
Source : E. Coli.
Supplied : Lyophilized protein from 10mM NaP, pH7.5 +0.1M L- Arg.
Purity : >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human TWEAK.
Reactivity : The ED50 as determined by the dose dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Tweak weakly induces the death of HT29 cells when cultured in hte presence of IFN-gamma. The Ed50 for this effect is between 30-45 ng/ml.
Reconstitution : The lyophilized TWEAK is soluble in water and most aqueous buffers. The lyophilized powder MUST be reconstituted in 10mM sodium phosphate, pH7.5 to a concentration of 1ug/ul. Allow to set at least 30 minutes at 4°C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4°Cfor up to 1 week or at -20°Cfor future use. For most in-vitro applications, TWEAk exerts its biological activity in the concentration range of 0.2 to 20.0 ng/ml.
Note : If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage : The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20°C. Reconstituted TWEAK should be stored in working aliquots at -20°C. Avoid frequent freeze thaw cycles.
Gene Name : TNFSF12 tumor necrosis factor (ligand) superfamily, member 12 [ Homo sapiens ]
Synonyms : TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; APO3L; DR3LG; TWEAK; MGC20669; MGC129581; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; Tumor necrosis factor ligand superfamily member 12; APO3 ligand; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; UNQ181/PRO207; MGC129581; MGC20669
Gene ID : 8742
mRNA Refseq : NM_003809
Protein Refseq : NP_003800
MIM : 602695
UniProt ID : O43508.
Chromosome Location : 17p13.1
Pathway : Cytokine-cytokine receptor interaction
Function : cytokine activity; tumor necrosis factor receptor binding

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