||Recombinant human Annexin A1 protein was expressed inE.coliand purified by using conventional chromatography. MW=38.7kDa.
||Annexin A1, also known as Lipocortin I, belongs to a family of Ca(2+)-dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. It promotes membrane fusion and is also involved in exocytosis. Since Annexin A1 has phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity, this protein may have potential anti-inflammatory activity. The detection of this protein by immunocytochemical means reportedly provides a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia.
|Amino Acid Sequence:
||MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKC ATSKPAFFAE KLHQAMKGVG TRHKALIRIM VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN.
||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl, 1mM DTT, 10% glycerol.
||> 90% by SDS–PAGE.
||1mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.