||Recombinant human Neurotrophin-4was expressed in E. coli.CTF1 is a dimer, formed by two identical 130 amino acid subunits, which is held together by strong hydrophobic interactions.
||Neurotrophin-4 (NT-4/NT-5) is a potent neutrophic factor which supports the growth and survivability of nerve and/or glial cells.
||GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA
||Lyophilized protein with no additives.
||>98% by SDS-Page analysis.Enodtoxin <0.1ng per ug (1EU/ug).
||The ED50 as determined by the dose dependent induction of choline acetyl transferase activity in RAT Basal Forebrain Septal Cultures was found to be 20-50ng/ml.
||The lyophilized human NT-4 is soluble in water and most aqueous buffers. The lyophilized powder hould be reconstituted in water to a concentration of 100 ng/ul.Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and stored at 4°Cofr up to 1 week or stored at -20°Cfor future use.
||The lyophilized powder, though stable at room temperature for a few weeks , but is best stored dessicated at-20℃. Reconstituted NT-4 should be stored in working aliquots at -20℃. Avoid Frequent Freeze-THAW Cycles.