Active Recombinant Human CA10 protein, Fc-His-tagged

• Cat.No.: CA10-257H
• Product Overview: Active Recombinant Human CA10 protein(NP_001076002.1) (Met 1-Asn 300) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-300 aa
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5.
• Bio-activity: Measured by its esterase activity. The specific activity is >50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
• Molecular Mass: The recombinant human CARPX/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 526 amino acids and migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions as predicted.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CA10 carbonic anhydrase X [ Homo sapiens ]
• Official Symbol: CA10
• Synonyms: CA10; carbonic anhydrase X; carbonic anhydrase-related protein 10; CA RPX; CARPX; HUCEP 15; CARP X; CA-RP X; cerebral protein 15; cerebral protein-15; carbonic anhydrase-related protein X; CA-RPX; HUCEP-15
• Gene ID: 56934
• mRNA Refseq: NM_001082533
• Protein Refseq: NP_001076002
• MIM: 604642
• UniProt ID: Q9NS85