Recombinant Human CEACAM6 protein, His-tagged CEACAM6-664H

Recombinant Human CEACAM6 protein, His-tagged

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Recombinant Human CEACAM6 protein, His-tagged

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Cat.No. : CEACAM6-664H
Product Overview : Recombinant Human CEACAM6 (Lys35-Gly320) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) is also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Lys35
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 32.0 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Lys35-Gly320
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CEACAM6-664H :

Human CEACAM-6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of CEACAM6-664H :

Immobilized Human CEACAM-6, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-8, His,Avitag with a linear range of 0.02-0.313 μg/mL (QC tested).

Gene Name : CEACAM6
Official Symbol : CEACAM6
Synonyms : CEACAM6; carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen); NCA; carcinoembryonic antigen-related cell adhesion molecule 6; CD66c; normal cross-reacting antigen; non-specific crossreacting antigen; CEAL
Gene ID : 4680
mRNA Refseq : NM_002483
Protein Refseq : NP_002474
MIM : 163980
UniProt ID : P40199

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