Recombinant Human TFPI protein, His-tagged TFPI-875H

Recombinant Human TFPI protein, His-tagged

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Cat.No. : TFPI-875H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human TFPI(NP_006278.1)(Met 1-Lys 282) fused with His tag at C-terminal was expressed in HEK293.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4
Bio-activity : Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
Molecular Mass : The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.
Protein length : Met 1-Lys 282
Endotoxin : < 1.0 eu per μg of the protein as determined by the LAL method.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade~-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : TFPI tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) [ Homo sapiens ]
Official Symbol : TFPI
Synonyms : TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); LACI; tissue factor pathway inhibitor; EPI; extrinsic pathway inhibitor; TFI; TFPI1; anti-convertin;
Gene ID : 7035
mRNA Refseq : NM_006287
Protein Refseq : NP_006278
MIM : 152310
UniProt ID : P10646
Chromosome Location : 2q32
Pathway : Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem; Syndecan-4-mediated signaling events, organism-specific biosystem;
Function : endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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