Recombinant Human TNFRSF10A

• Cat.No.: TNFRSF10A-112H
• Product Overview: Human sTRAIL Receptor-1, also known as DR4 (Death Receptor-4), is a member of the TNF receptor family. This Human sTRAIL Receptor-1 is a 22.7 kDa protein containing 215 amino acid residues comprising the extracellular domain of TRAIL Receptor-1. It was produced inE.coli.

Specification

• Cat. No.: TNFRSF10A-112H
• Description: CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene.
• Source: E.coli.
• Amino Acid Sequence: MSGTGAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASQQLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHN.
• Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
• Supplied: Lyophilized protein.
• Purity: >95% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug).
• Reconstitution: The lyophilized protein soluble in water and most aqueous buffers. The lyophilized powder should first be quick spun in vial, then reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4℃. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial.
• Biological Activity: Determined by its inhibitory effect of IL-8 production in human PBMC by human sTRAIL/Apo2L. 95% inhibition (100 ng/ml of s TRAIL/Apo2L) was reached using a concentration of 100 ng/ml of sTRAIL Receptor-1.
• Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20℃. Reconstituted sTrail Receptor-1 should be stored in working aliquots at -20℃. Avoid Frequent Freeze-THAW Cycles.

Gene Information

• Gene Name: TNFRSF10A tumor necrosis factor receptor superfamily, member 10a [ Homo sapiens ]
• Synonyms: TNFRSF10A; tumor necrosis factor receptor superfamily, member 10a; DR4; APO2; CD261; MGC9365; TRAILR1; TRAILR-1; death receptor 4; cytotoxic TRAIL receptor; TNF-related apoptosis inducing ligand receptor 1; TRAIL receptor 1; TRAIL-R1
• Gene ID: 8797
• mRNA Refseq: NM_003844
• Protein Refseq: NP_003835
• MIM: 603611
• UniProt ID: O00220
• Chromosome Location: 8p21
• Pathway: Cytokine-cytokine receptor interaction; Natural killer cell mediated cytotoxicity; Apoptosis
• Function: TRAIL binding; caspase activator activity; death receptor activity; receptor activity; transcription factor binding