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Recombinant Human LGALS1

Cat. No.: LGALS1-669H
Product Overview: Recombinant human Galectin is a 14.5 kDa protein consisting of 134 amino acid residues. It was expressed inE. Coli.
Description: Galectin-1 belongs to growing family of evolutionary conserved animal lectins. Galectins consists of beta-galactoside binding lectins that contain homologous carbohydrate recognition domains (CRDs). Galectin-1 has the ability to induce apoptosis of activated T-cells and T-leukaemia cell lines. Other activities include cell differentiation and inhibition activity of CD45 protein phosphatase activity. Galectin also binds beta-galactoside as well as CD45, CD3 and CD4.
Source: Escherichia Coli.
Amino Acid Sequence: ACGLVASNL NLKPGQCLRV RGQVAPDAKSFVLNLGKDSN NLCLHFNPRF NAHGDANTIVCNSKDGGAWG TQERQAVFPF EPGSVAQVCITFDEANLTVK LPDGYQFKFP NRLNLQAINYMAADGDFKIK CVAFD.
Supplied: The sterile filtered solution was lyophilized from 10mM Sodium Phosphate, pH 7.5.
Purity: Greater than 97% by SDS-PAGE. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Reconstitution: The lyophilized powder is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water or 20mM Tris pH7.5 to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4°C for up to 1 week or at -20°C for future use.
Note: If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Maintain stock >10ug/ml.
Reactivity: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concnetration of 2.5ug/ml with a peak response obtained at 250ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted human galectin-1 should be stored in working aliquots at -20 °C. avoid frequent freeze thaw cycles.
Gene Name: LGALS1 lectin, galactoside-binding, soluble, 1 [ Homo sapiens ]
Synonyms: lectin, galactoside-binding, soluble, 1; GBP; GAL1; DKFZp686E23103; LGALS1; galectin-1; galaptin; galectin 1; S-Lac lectin 1; lactose-binding lectin 1; Galaptin; HBL; HPL; beta-galactoside-binding lectin L-14-I; OTTHUMP00000028982; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12
Gene ID: 3956
mRNA Refseq: NM_002305
Protein Refseq: NP_002296
MIM: 150570
UniProt ID: P09382
Chromosome Location: 22q13.1
Function: protein homodimerization activity; galactoside binding; glycoprotein binding; signal transducer activity; sugar binding

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