Recombinant Human TNFRSF1B

• Cat.No.: TNFRSF1B-180H
• Product Overview: Recombinant human sTNFRII produced inE.coliis an 18.9 kDa protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFRII protein.

Specification

• Cat. No.: TNFRSF1B-180H
• Description: TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-α but manifests a lower affinity to TNF-β. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-α induced activities by acting as a decoy receptor.
• Source: E.coli.
• Purity: > 98% by SDS-PAGE and HPLC analyses.
• Endotoxin Level: < 0.1 ng per ug of sTNF-receptor.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biological Activity: Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED₅₀for this effect in the presence of 0.25 ng/ml of recombinant human TNF-α, is 0.125 μg/ml.
• Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20℃ for future use.
• Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20℃. Reconstituted sTNFRII is stable for at least 3 months when stored in working aliquots with a carrier protein at -20℃. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TNFRSF1B tumor necrosis factor receptor superfamily, member 1B [ Homo sapiens ]
• Synonyms: TNFRSF1B; tumor necrosis factor receptor superfamily, member 1B; Tumour necrosis factor receptor 2; Tumour receptor beta receptor; Tumour necrosis factor binding protein 2; Tumour necrosis factor receptor superfamily member 1B; Tumour necrosis factor receptor type II; p80 TNF alpha; receptor; Etanercept; CD120b; p75; TBPII; TNFRII, TNFR75; TNFBR; TNFR2; TNFR80; TNFR1B; TNF-R75; p75TNFR; TNF-R-II; TNFRSF1B; p75 TNF receptor; OTTHUMP00000001784; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; TNF-R2; CD120b antigen
• Gene ID: 7133
• mRNA Refseq: NM_001066
• Protein Refseq: NP_001057
• MIM: 191191
• UniProt ID: P20333
• Chromosome Location: 1p36.3-p36.2
• Function: protein binding; receptor activity; tumor necrosis factor receptor activity