||Recombinant Human epiregulin produced inE.coliwasa 5.6kDa monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence.
||Epiregulin is an EGF related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes and in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0kDa mature secreted sequence.
||>95% by SDS-PAGE and HPLC analysis.
||<0.1ng/μg of Epiregulin.
||The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is ≤2.0ng/ml, corresponding to a specific activity of ≥5x105 units/mg.
||The lyophilized protein is stable for at least 2 years from date of receipt at -20℃. Reconstituted Epiregulin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20℃